As part of an active scientific community that studies the DNA damage response (DDR) and its impact on cancer and aging, research in the lab of Matthias Altmeyer is aimed at elucidating cellular mechanisms of genome integrity maintenance and their deregulation in human disease. Here you can explore who we are and what we do. Welcome!

TRIP12 protects from PARPi-induced PARP trapping

Reduced operating mode continues due to COVID-19 situation

As the University of Zurich resumes on-site operations with applicable safety concepts in place also our lab resumes experimental work, albeit with reduced capacity and with safety precautions.

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TRIP12 protects from PARPi-induced PARP trapping

TRIP12 protects from PARPi-induced PARP trapping

In our recent publication in Cell Reports we reveal that the ubiquitin E3 ligase TRIP12, through its PAR-binding WWE domain, targets PARP1 for proteasomal degradation, affecting PARP functions and PARPi efficiency.

Publications

Postdoctoral Candidates and MSc/PhD Students

Postdoctoral Candidates and MSc/PhD Students

We have openings for a postdoctoral researcher and MSc/PhD students to join our lab. If you are interested in cancer biology and genome integrity maintenance and have a liking for quantitative cell biology, please take a look.

Current Openings

Featured News


Marco’s paper on TRIP12-regulated PARP inhibitor efficiency is out

August 2020

The ubiquitin ligase TRIP12 limits PARP1 trapping and constrains PARP inhibitor efficiency

We show that the ubiquitin E3 ligase TRIP12, through its PAR-binding WWE domain, targets PARP1 for proteasomal degradation, affecting PARP functions and PARP inhibitor efficiency. Great work with important implications worth following up. Congratulations to Marco Gatti and big thanks to our collaborators Qingyao Huang and Michael Baudis for their help. Published in Cell Reports.

Highlights:

  • TRIP12 is a PAR-targeted ubiquitin ligase (PTUbL)
  • The HECT and WWE domains of TRIP12 cooperate to mediate PARP1 turnover
  • TRIP12 loss sensitizes cancer cells to PARP inhibitors in a PARP1-dependent manner
  • TRIP12 status may help to estimate how cancer cells respond to PARP inhibition

Further reading:

Gatti et al., Cell Rep. 2020 Aug 4;32(5):107985.


Matteo’s and Federico’s collaborative paper is out

July 2019

Spearheaded by Matteo Berti from Massimo Lopes’ group and Federico Teloni from our lab, our collaborative study on RAD51 and replication fork reversal is finally out in Nature Communications.

Sequential role of RAD51 paralog complexes in replication fork remodeling and restart

Further reading:

Berti, Teloni et al., Nat Commun. 2020 Jul 15;11(1):3531.


DNA repair enters a new liquid phase

August 2019

The DNA damage response factor and chromatin binder 53BP1 generates repair compartments around DNA breaks. In a paper published in EMBO Journal (2019)38:e101379 we show that these nuclear compartments show liquid-like properties, exhibit induced self‐assembly based on 53BP1’s oligomerization potential, and undergo fusion and fission events with potential implications for break clustering and chromosomal translocations. 53BP1 condensates are sensitive to disruption of weak interactions, and can co-assemble and contribute to the activation of tumor suppressor protein p53. See also the News & Views by Piccinno et al.

Phase separation of 53BP1 determines liquid-like behavior of DNA repair compartments

Highlights:

  • DNA repair compartments show liquid‐like properties with droplet fusions and fissions
  • Phase separation behavior is conferred by 53BP1, and uncoupled from upstream DNA damage detection and γH2AX‐MDC1 accumulation
  • 53BP1 assembly at break sites is abolished by changes in osmotic pressure, temperature, salt concentration, or disruption of hydrophobic interactions
  • 53BP1 optoDroplet experiments indicate multivalency and reveal sequence elements required for clustering
  • Disruption of 53BP1 phase separation impairs p53 activation and p21 induction upon DNA damage

Further reading:

Kilic et al., EMBO J (2019)38:e101379


Article published in Molecular Cell

February 2019

Replication stress is a hallmark of many cancers. In an article published in the February 2019 issue of Molecular Cell (Volume 73, Issue 4, p641-858) we identify the pre-mRNA cleavage factor WDR33 as regulator of replication stress resilience and demonstrate that, when WDR33 function is impaired, unreleased nascent transcripts and genomic loci re-localize toward the nuclear periphery, where they cause replication stress and DNA damage.

Efficient pre-mRNA cleavage prevents replication-stress-associated genome instability

Highlights:

  • A convergent multi-screening approach reveals modulators of replication stress (RS)
  • Efficient WDR33-mediated pre-mRNA cleavage confers RS resilience
  • RNA:DNA-hybrid formation occurs upon RS-induced DNA breakage
  • THO nuclear export complex drives RS sensitivity of WDR33-depleted cells

Further reading:

Teloni et al. Mol Cell. 2019 Feb 21;73(4):670-683.e12


Measuring cellular responses to PARP inhibition

July 2018

Our paper on PARP inhibitor toxicity is published in Nature Communications! Have a look how high-content imaging reveals the earliest cellular responses to PARP inhibition and unravels the sequence of events from PARP trapping to DNA damage, cell cycle arrest and cell death. Here you can access the complete article and browse through the Editors’ Highlights, or read the UZH/EurekAlert public releases.

Analysis of PARP inhibitor toxicity by multidimensional fluorescence microscopy reveals mechanisms of sensitivity and resistance

Further reading:

Michelena et al. Nat Commun. 2018 Jul 11;9(1):2678.


Lab research featured in UZH Magazin and in the Annual Report 2017

March 2018

Research of the group brought to life by Roland Fischer and photographer Marc Latzel for UZH Magazin 01/2018 (UZH News). Download the full article or the complete magazine (in German). Read the English translation here.

Our work was also included in the UZH Annual Report 2017. Download the complete Annual Report or have a look at the online summary.


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